Abstract
The analysis of cancer cell behavior in the primary tumor in living animals provides an opportunity to explore the process of invasion and intravasation in the complex microenvironment that is present in vivo. In this chapter, we describe the methods that we have developed for performing intravital imaging of mammary tumors. We provide procedures for generating tumors through injection of tumor cell lines, and multiphoton imaging using a skin-flap tumor dissection and a mammary imaging window.
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Acknowledgments
The work described herein was supported by CA100324, CA113395, and CA126511 (B.G., D.E., J.W., and J.C.) and CA100324 and CA77522 (J.H., D.K., and J.E.S.).
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Hulit, J. et al. (2012). The Use of Fluorescent Proteins for Intravital Imaging of Cancer Cell Invasion. In: Hoffman, R. (eds) In Vivo Cellular Imaging Using Fluorescent Proteins. Methods in Molecular Biology, vol 872. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-797-2_2
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DOI: https://doi.org/10.1007/978-1-61779-797-2_2
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