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Establishment of hESC Lines from the Inner Cell Mass of Blastocyst-Stage Embryos and Single Blastomeres of 4-Cell Stage Embryos

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Human Embryonic Stem Cells Handbook

Part of the book series: Methods in Molecular Biology ((MIMB,volume 873))

Abstract

More than 600 human embryonic stem cell (hESC) lines have been reported today at the human European Embryonic Stem Cell Registry (http://www.hescreg.eu/). Despite these high numbers, there are currently no general protocols for derivation, culture, and characterization of hESC. Moreover, data on the culture of the embryo used for the derivation (medium, day of ICM isolation) are usually not available but can have an impact on the derivation rate. We present here the protocols for derivation, culture and characterization as we applied them for the 22 hESC lines (named VUB-hESC) in our laboratory.

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Acknowledgments

We gratefully acknowledge the assistance of Urielle Ullmann, Nele De Temmermann, Lindsey Van Haute, Bing Chen, Anna Seriola Petit, Claudia Spits, and Rogier Schellens for deriving and maintaining the VUB-hESC lines in culture.

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Authors and Affiliations

  1. Research Group Reproduction and Genetics, Vrije Universiteit Brussel, Brussels, Belgium

    Ileana Mateizel, Mieke Geens, Hilde Van de Velde & Karen Sermon

Authors
  1. Ileana Mateizel
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  2. Mieke Geens
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  3. Hilde Van de Velde
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  4. Karen Sermon
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Corresponding author

Correspondence to Ileana Mateizel .

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Editors and Affiliations

  1. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Smyth Road 501, Ottawa, K1Y 8L6, Canada

    Kursad Turksen

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Mateizel, I., Geens, M., Van de Velde, H., Sermon, K. (2012). Establishment of hESC Lines from the Inner Cell Mass of Blastocyst-Stage Embryos and Single Blastomeres of 4-Cell Stage Embryos. In: Turksen, K. (eds) Human Embryonic Stem Cells Handbook. Methods in Molecular Biology, vol 873. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-794-1_6

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  • DOI: https://doi.org/10.1007/978-1-61779-794-1_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-793-4

  • Online ISBN: 978-1-61779-794-1

  • eBook Packages: Springer Protocols

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