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Derivation, Propagation, and Characterization of Neuroprogenitors from Pluripotent Stem Cells (hESCs and hiPSCs)

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Human Embryonic Stem Cells Handbook

Part of the book series: Methods in Molecular Biology ((MIMB,volume 873))

Abstract

The differentiation of human embryonic stem cells (hESCs) and human-induced pluripotent stem cells (hiPSCs) towards functional neurons particularly hold great potential for the cell-based replacement therapy in neurodegenerative diseases. Here, we describe a stepwise differentiation protocol that mimics the early stage of neural development in human to promote the generation of neuroprogenitors at a high yield. Both the hESCs and hiPSCs are initially cultured in an optimized feeder-free condition, which offer an efficient formation of aggregates. To specify the neuroectodermal specification, these aggregates are differentiated in a defined neural induction medium to develop into neural rosettes-like structures. The rosettes are expanded into free-floating sphere and can be further propagated or developed into variety of neuronal subtypes.

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Acknowledgment

This research was supported by the Stem cell Initiative of the Faculty of Medicine, University of New South Wales, Australia.

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Authors and Affiliations

  1. Stem Cell Laboratory, Faculty of Medicine, School of Psychiatry, University of New South Wales, Randwick, NSW, Australia

    Khun-Hong Lie, Henry C. Y. Chung & Kuldip S. Sidhu

Authors
  1. Khun-Hong Lie
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  2. Henry C. Y. Chung
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  3. Kuldip S. Sidhu
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Corresponding author

Correspondence to Kuldip S. Sidhu .

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Editors and Affiliations

  1. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Smyth Road 501, Ottawa, K1Y 8L6, Canada

    Kursad Turksen

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Lie, KH., Chung, H.C.Y., Sidhu, K.S. (2012). Derivation, Propagation, and Characterization of Neuroprogenitors from Pluripotent Stem Cells (hESCs and hiPSCs). In: Turksen, K. (eds) Human Embryonic Stem Cells Handbook. Methods in Molecular Biology, vol 873. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-794-1_15

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  • DOI: https://doi.org/10.1007/978-1-61779-794-1_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-793-4

  • Online ISBN: 978-1-61779-794-1

  • eBook Packages: Springer Protocols

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