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Antisense-Mediated Exon-Skipping to Induce Gene Knockdown

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Exon Skipping

Part of the book series: Methods in Molecular Biology ((MIMB,volume 867))

Abstract

Exon-skipping antisense oligonucleotides (ASOs) can be used to knockdown the expression of an undesired gene or specific gene isoform. This chapter discusses the potential therapeutic applications of the technique and provides a sample protocol for inducing exon-skipping in Apolipoprotein B in vitro, as well as a protocol for quantifying exon-skipping using real-time PCR.

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Authors and Affiliations

  1. Department of Endocrinology, UCL Medical School, London, UK

    Petra Disterer & Bernard Khoo

Authors
  1. Petra Disterer
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  2. Bernard Khoo
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Corresponding author

Correspondence to Bernard Khoo .

Editor information

Editors and Affiliations

  1. Dept. Human & Clinical Genetics, Leiden University Medical Center, Einthovenweg 20, Leiden, 2333 ZC, Netherlands

    Annemieke Aartsma-Rus

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Disterer, P., Khoo, B. (2012). Antisense-Mediated Exon-Skipping to Induce Gene Knockdown. In: Aartsma-Rus, A. (eds) Exon Skipping. Methods in Molecular Biology, vol 867. Humana Press. https://doi.org/10.1007/978-1-61779-767-5_19

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  • DOI: https://doi.org/10.1007/978-1-61779-767-5_19

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-766-8

  • Online ISBN: 978-1-61779-767-5

  • eBook Packages: Springer Protocols

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