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Isolation of Microbial Natural Products

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Natural Products Isolation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 864))

Abstract

In principle, the isolation of secondary metabolites from microbes does not differ from their isolation from other organisms. The extraction procedure may of course be quite different, especially if it is carried out in an industrial scale, but when an extract containing the metabolites of interest is at hand, it is the same palette of adsorbents and chromatographic techniques that provide the major tools for the fractionation and eventual isolation of the pure compounds. Compared to plants, in which one is sure to find secondary metabolites of certain types, e.g., flavonoids, microbes can be expected to produce virtually anything and it is important to go about the fractionation procedure with an open mind. This chapter presents an overview of preparation of extracts from microbial sources, and various methods and strategies involved in the isolation and characterization of microbial natural products.

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Author information

Authors and Affiliations

  1. Department of Chemistry, Lund University, Lund, Sweden

    Olov Sterner

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  1. Olov Sterner
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Correspondence to Olov Sterner .

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Editors and Affiliations

  1. School of Applied Sciences, Department of Pharmacy, University of Wolverhampton, Wulfruna Street, Wolverhampton, WV1 1LY, United Kingdom

    Satyajit D. Sarker

  2. , Leicester School of Pharmacy, De Montfort University, The Gateway, Leicester, LE1 9BH, United Kingdom

    Lutfun Nahar

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Sterner, O. (2012). Isolation of Microbial Natural Products. In: Sarker, S., Nahar, L. (eds) Natural Products Isolation. Methods in Molecular Biology, vol 864. Humana Press. https://doi.org/10.1007/978-1-61779-624-1_15

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  • DOI: https://doi.org/10.1007/978-1-61779-624-1_15

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-623-4

  • Online ISBN: 978-1-61779-624-1

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