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Influenza Virus pp 163–174Cite as

Genetic Engineering of Live Attenuated Influenza Viruses

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 865))

Abstract

The first live attenuated influenza vaccine (LAIV) was licensed in the USA in 2003; it is a trivalent vaccine composed of two type A (H1N1 and H3N2) and one type B influenza virus each at 107 fluorescent focus units (FFU). Each influenza vaccine strain is a reassortant virus that contains the hemagglutinin (HA) and neuraminidase (NA) gene segments from a wild-type influenza virus and the six internal protein gene segments from a master donor virus (MDV) of either cold-adapted A/Ann Arbor/6/60 or B/Ann Arbor/1/66. MDV confers the cold-adapted, temperature-sensitive, and attenuation phenotypes to the vaccine strains. The reassortant vaccine seeds are currently produced by reverse genetics and amplified in specific pathogen-free (SPF) 9–11 days old embryonated chicken eggs for manufacture. In addition, MDCK cell culture manufacture processes have been developed to produce LAIV for research use and with modifications for clinical and/or commercial grade material production.

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Authors and Affiliations

  1. MedImmune, Mountain View, CA, USA

    Hong Jin, Zhongying Chen, Jonathan Liu & George Kemble

Authors
  1. Hong Jin
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  2. Zhongying Chen
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  3. Jonathan Liu
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  4. George Kemble
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Corresponding author

Correspondence to Hong Jin .

Editor information

Editors and Affiliations

  1. School of Veterinary Medicine, Dept. Pathobiological Science, University of Wisconsin-Madison, Science Drive 575, Madison, 53711, Wisconsin, USA

    Yoshihiro Kawaoka

  2. Influenza Research Institute (IRI), Dept. Pathobiological Science, University of Wisconsin-Madison, Science Drive 575, Madison, 53711, Wisconsin, USA

    Gabriele Neumann

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© 2012 Springer Science+Business Media, LLC

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Jin, H., Chen, Z., Liu, J., Kemble, G. (2012). Genetic Engineering of Live Attenuated Influenza Viruses. In: Kawaoka, Y., Neumann, G. (eds) Influenza Virus. Methods in Molecular Biology, vol 865. Humana Press. https://doi.org/10.1007/978-1-61779-621-0_10

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  • DOI: https://doi.org/10.1007/978-1-61779-621-0_10

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-620-3

  • Online ISBN: 978-1-61779-621-0

  • eBook Packages: Springer Protocols

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