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Introduction to Metabolomics

  • Teresa Whei-Mei FanEmail author
  • Andrew N. Lane
  • Richard M. Higashi
Protocol
Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Abstract

We provide an overview of metabolomics in its current practices, including sample processing, major techniques, example applications, and useful resources to the readership, as described in detail in the various chapters of the handbook. In our view, metabolomics can be defined as “a systematic interrogation of the metabolome in terms of metabolite concentration, structure, and transformation pathways.” We emphasize the use of stable isotope tracing methodologies both in vitro and in vivo. We further provide a brief introduction to some concepts of flux analyses at steady state and nonsteady state.

Key words

Stable isotope editing Metabolic pathways Flux analysis 

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Teresa Whei-Mei Fan
    • 1
    Email author
  • Andrew N. Lane
    • 2
  • Richard M. Higashi
    • 1
  1. 1.Department of Chemistry, Center for Regulatory and Environmental Analytical Metabolomics (CREAM), and James Graham Brown Cancer CenterUniversity of LouisvilleLouisvilleUSA
  2. 2.Departments of Medicine and Chemistry, Center for Regulatory and Environmental Analytical Metabolomics (CREAM), and James Graham Brown Cancer CenterUniversity of LouisvilleLouisvilleUSA

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