Abstract
Methylated CpG island amplification and microarray (MCAM) is a two-color array technique that quantifies DNA methylation by hybridizing equimolar amounts of treated vs. control cell-line DNA to an array platform. Sample preparation, hybridization, and scanning are performed over 1 week, but multiple samples can be prepared simultaneously, allowing for high-throughput processing and data acquisition. One microarray slide is required for each sample assayed, with a control sample hybridized with each test sample.
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Colyer, H.A.A., Dellett, M., Mills, K.I. (2012). Detecting DNA Methylation Using the Methylated CpG Island Amplification and Microarray Technique. In: Dumitrescu, R., Verma, M. (eds) Cancer Epigenetics. Methods in Molecular Biology, vol 863. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-612-8_21
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DOI: https://doi.org/10.1007/978-1-61779-612-8_21
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-61779-612-8
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