Skip to main content
SpringerLink
Log in

Microarray for Epigenetic Changes: Gene Expression Arrays

  • Protocol
  • First Online:
Cancer Epigenetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 863))

Abstract

Microarrays can be used to examine changes involving all aspects of the epigenetic interactions. As the relationship between DNA methylation, histone modifications, and gene expression is elucidated, an important aspect to investigate is how the epigenetic status regulates the cell through differential expression of genes. This can provide data from work including experimental drug investigations from cell line models, or more directly patient comparison data.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Fodor, S. P., Rava, R. P., Huang, X. C., Pease, A. C., Holmes, C. P., and Adams, C. L. (1993) Multiplexed biochemical assays with biological chips, Nature 364, 555–556.

    Article  PubMed  CAS  Google Scholar 

  2. Glas, A. M., Floore, A., Delahaye, L. J., Witteveen, A. T., Pover, R. C., Bakx, N., Lahti-Domenici, J. S., Bruinsma, T. J., Warmoes, M. O., Bernards, R., Wessels, L. F., and Van’t Veer, L. J. (2006) Converting a breast cancer microarray signature into a high-throughput diagnostic test, BMC Genomics 7, 278.

    Article  PubMed  Google Scholar 

  3. Gutman, S. I. (2004) Evaluation of Automatic Class III Designation Roche AmpliChip CYP450 Test, (Administration, F. a. D., Ed.), pp 1–3, Department of Health and Human Services, Rockville MD.

    Google Scholar 

  4. Li, X., Quigg, R. J., Zhou, J., Gu, W., Nagesh Rao, P., and Reed, E. F. (2008) Clinical utility of microarrays: current status, existing challenges and future outlook, Curr Genomics 9, 466–474.

    Article  PubMed  CAS  Google Scholar 

  5. Affymetrix. (2004) Data Sheet GeneChip Human Genome Arrays, Santa Clara.

    Google Scholar 

  6. Affymetrix. (2002) GeneChip Expression Analysis: Technical Manual (P/N 701021 rev 1).

    Google Scholar 

  7. Affymetrix. (2009) GeneChip Expression Analysis: Technical Manual With Specific Protocols for Using the GeneChip Hybridization, Wash, and Stain Kit (P/N 702232 Rev. 3).

    Google Scholar 

  8. Schroeder, A., Mueller, O., Stocker, S., Salowsky, R., Leiber, M., Gassmann, M., Lightfoot, S., Menzel, W., Granzow, M., and Ragg, T. (2006) The RIN: an RNA integrity number for assigning integrity values to RNA measurements, BMC Mol Biol 7, 3.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Haematology, Centre for Cancer Research and Cell Biology Belfast, Queen’s University Belfast, Belfast, Northern Ireland, UK

    Hilary A. A. Colyer & Ken I. Mills

  2. Haematology Center for Cancer Research and Cell Biology Belfast, Queen’s University Belfast, Belfast, Northern Ireland, UK

    Richard N. Armstrong

Authors
  1. Hilary A. A. Colyer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Richard N. Armstrong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ken I. Mills
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hilary A. A. Colyer .

Editor information

Editors and Affiliations

  1. School of Medicine, Saba University, Saba, Dutch Antilles

    Ramona G. Dumitrescu

  2. National Institutes of Health (NIH), Div. Cancer Control &, National Cancer Institute, Executive Blvd. 6130Suite 5100, Bethesda, 20892-7324, Maryland, USA

    Mukesh Verma

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Colyer, H.A.A., Armstrong, R.N., Mills, K.I. (2012). Microarray for Epigenetic Changes: Gene Expression Arrays. In: Dumitrescu, R., Verma, M. (eds) Cancer Epigenetics. Methods in Molecular Biology, vol 863. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-612-8_20

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-612-8_20

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-611-1

  • Online ISBN: 978-1-61779-612-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation