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Cancer Epigenetics pp 303–317Cite as

Genome-Wide Methylation Analysis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 863))

Abstract

The disruption and alteration of genomic methylation patterns is a hallmark of cancer and other disease states. Understanding and characterizing genome-wide methylation will have a profound effect on our understanding of tumorigenesis and provide novel avenues for therapy. This chapter serves to describe techniques that examine genome-wide methylation patterns including luminometric methylation assay, restriction landmark genome scanning, and the cytosine extension assay, which utilize methylation-sensitive restriction enzymes. Additional techniques such as nucleotide separation assays (nearest neighbor analysis and high-performance capillary electrophoresis) and the infinium methylation assay are discussed. These techniques allow for the determination of changes in global methylation levels, as well as regional changes in methylation throughout the genome.

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Author information

Authors and Affiliations

  1. Division of Neurosurgery, The Hospital for Sick Children, Toronto, ON, Canada

    Alexander Unterberger

  2. Department of Laboratory Medicine and Pathobiology, Division of Neurosurgery, University of Toronto, Toronto, ON, Canada

    Adrian M. Dubuc & Michael D. Taylor

Authors
  1. Alexander Unterberger
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  2. Adrian M. Dubuc
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  3. Michael D. Taylor
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Corresponding author

Correspondence to Alexander Unterberger .

Editor information

Editors and Affiliations

  1. School of Medicine, Saba University, Saba, Dutch Antilles

    Ramona G. Dumitrescu

  2. National Institutes of Health (NIH), Div. Cancer Control &, National Cancer Institute, Executive Blvd. 6130Suite 5100, Bethesda, 20892-7324, Maryland, USA

    Mukesh Verma

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Unterberger, A., Dubuc, A.M., Taylor, M.D. (2012). Genome-Wide Methylation Analysis. In: Dumitrescu, R., Verma, M. (eds) Cancer Epigenetics. Methods in Molecular Biology, vol 863. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-612-8_19

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  • DOI: https://doi.org/10.1007/978-1-61779-612-8_19

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-611-1

  • Online ISBN: 978-1-61779-612-8

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