Restriction Fragment Length Polymorphism of the 5S-rRNA-NTS Region: A Rapid and Precise Method for Plant Identification

  • Cinzia Margherita BerteaEmail author
  • Giorgio Gnavi
Part of the Methods in Molecular Biology book series (MIMB, volume 862)


Molecular genetic methods have several advantages over classical morphological and chemical analyses. The genetic method requires genotype instead than phenotype, therefore PCR-based techniques have been widely used for a rapid identification of plant species, varieties and chemotypes. Recently, the molecular discrimination of some higher plant species has been evaluated using sequences of a 5S-rRNA gene spacer region. The variation in the nontranscribed sequence (NTS) region has been used in a number of plant species for studying intraspecific variation, genome evolution, and phylogenetic reconstruction. Here, we describe a rapid method based on the use of the 5S-rRNA-NTS region as a tool for plant DNA fingerprinting, which combines PCR, sequencing and restriction fragment length polymorphism analyses.

Key words

DNA fingerprinting 5S-rRNA gene Nontranscribed sequence region PCR–RFLP High-resolution gel capillary electrophoresis Agilent 2100 Bioanalyzer 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Plant Physiology Unit, Department of Plant BiologyUniversity of TurinTurinItaly

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