DNA Purification from Multiple Sources in Plant Research with Homemade Silica Resins
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DNA purification is a routine procedure in most plant laboratories. Although different kits are available in the market allowing convenient DNA purification, the cumulative cost of purchasing multiple kits for a laboratory can be staggering. Here, we describe a protocol using homemade silicon dioxide matrix for DNA purification from Escherichia coli and Agrobacterium tumefaciens cells, PCR and restriction digestion mixtures, agarose gel slices and plant tissues. Compared with the commercial kits, this protocol enables easy DNA purification from diverse sources with comparable yield and purity at negligible expenses.
Key wordsDNA purification Silicon dioxide Escherichia coli Agrobacterium tumefaciens PCR product Agarose gel Plant tissue
This work was supported by NIH R01 grant GM070567 to J.S. and a postdoctoral fellowship award from the MGH fund for medical discovery to J.F.L.
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