Advertisement

DNA Purification from Multiple Sources in Plant Research with Homemade Silica Resins

  • Jian-Feng LiEmail author
  • Jen Sheen
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 862)

Abstract

DNA purification is a routine procedure in most plant laboratories. Although different kits are available in the market allowing convenient DNA purification, the cumulative cost of purchasing multiple kits for a laboratory can be staggering. Here, we describe a protocol using homemade silicon dioxide matrix for DNA purification from Escherichia coli and Agrobacterium tumefaciens cells, PCR and restriction digestion mixtures, agarose gel slices and plant tissues. Compared with the commercial kits, this protocol enables easy DNA purification from diverse sources with comparable yield and purity at negligible expenses.

Key words

DNA purification Silicon dioxide Escherichia coli Agrobacterium tumefaciens PCR product Agarose gel Plant tissue 

Notes

Acknowledgment

This work was supported by NIH R01 grant GM070567 to J.S. and a postdoctoral fellowship award from the MGH fund for medical discovery to J.F.L.

References

  1. 1.
    Boom, R., Sol, C.J., Salimans, M.M., Jansen, C.L., Wertheim-van Dillen, P.M., and Noordaa van der, J. (1990) Rapid and simple method for purification of nucleic acids. J Clin Microbiol 28, 495–503.PubMedGoogle Scholar
  2. 2.
    Li, J.F., Li, L., and Sheen, J. (2010) Protocol: a rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology. Plant Methods 6Google Scholar
  3. 3.
    Boyle, J.S. and Lew, A.M. (1995) An inexpensive alternative to glassmilk for DNA purification. Trends Genet 11, 8.PubMedCrossRefGoogle Scholar
  4. 4.
    Yoo, S.D., Cho, Y.H., and Sheen, J. (2007) Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protoc 2: 1565–1572.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Molecular BiologyMassachusetts General HospitalBostonUSA
  2. 2.Department of GeneticsHarvard Medical SchoolBostonUSA

Personalised recommendations