Multiplex PCR Method to Discriminate Artemisia iwayomogi from Other Artemisia Plants
- 2k Downloads
Some plants in the genus Artemisia have been used for medicinal purposes. Among them, Artemisia iwayomogi, commonly referred to as “Haninjin,” is one of the major medicinal materials used in traditional Korean medicine. By contrast, Artemisia capillaris and both Artemisia argyi and Artemisia princeps, referred to as “Injinho” and “Aeyup,” respectively, are used to treat diseases different from those for which “Haninjin” is prescribed. Therefore, the development of a reliable method to differentiate each Artemisia herb is necessary. We found that a random amplified polymorphic DNA (RAPD) method can be used to efficiently discriminate a few Artemisia plants from one another. To improve the reliability of RAPD amplification, we designed primer sets based on the nucleotide sequences of RAPD products to amplify a sequence-characterized amplified region (SCAR) marker of A. iwayomogi. In addition, we designed two other primer sets to amplify SCAR markers of “Aeyup” (A. argyi and A. princeps) along with “Injinho” (A. capillaris) and Artemisia japonica, which are also traded in Korean herbal markets. Using these three primer sets, we developed a multiplex PCR method concurrently not only to discriminate A. iwayomogi from other Artemisia plants, but also to identify Artemisia plants using a single PCR process.
Key wordsArtemisia plants Random amplified polymorphic DNA Sequence-characterized amplified region marker Multiplex PCR
- 1.Korea Food & Drug Administration (2002) The Korean Herbal Pharmacopoeia. Korea Food & Drug Administration, SeoulGoogle Scholar
- 2.McClelland M, Welsh J (1994) DNA fingerprinting by arbitrarily primed PCR. PCR Methods Appl. 4:59–65Google Scholar
- 3.Mi Young Lee, Eui Jeong Doh, Chae Haeng Park, Young Hwa Kim, Eung Soo Kim, Byong Seob Ko and Seung-Eun Oh (2006) Development of SCAR Marker for Discrimination of Artemisia princeps and A. argyi from Other Artemisia Herbs. Biol. Pharm.Bull. 29(4):629–633Google Scholar
- 5.Burgart, L., Robinson, R., Heller, M., Wilke, W., Iakoubova, O., and Chevill, J. (1992) Multiplex polymerase chain reaction. Modern Pathol., 5:320–323Google Scholar