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Multiplex PCR Method to Discriminate Artemisia iwayomogi from Other Artemisia Plants

  • Eui Jeong Doh
  • Seung-Eun OhEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 862)

Abstract

Some plants in the genus Artemisia have been used for medicinal purposes. Among them, Artemisia iwayomogi, commonly referred to as “Haninjin,” is one of the major medicinal materials used in traditional Korean medicine. By contrast, Artemisia capillaris and both Artemisia argyi and Artemisia princeps, referred to as “Injinho” and “Aeyup,” respectively, are used to treat diseases different from those for which “Haninjin” is prescribed. Therefore, the development of a reliable method to differentiate each Artemisia herb is necessary. We found that a random amplified polymorphic DNA (RAPD) method can be used to efficiently discriminate a few Artemisia plants from one another. To improve the reliability of RAPD amplification, we designed primer sets based on the nucleotide sequences of RAPD products to amplify a sequence-characterized amplified region (SCAR) marker of A. iwayomogi. In addition, we designed two other primer sets to amplify SCAR markers of “Aeyup” (A. argyi and A. princeps) along with “Injinho” (A. capillaris) and Artemisia japonica, which are also traded in Korean herbal markets. Using these three primer sets, we developed a multiplex PCR method concurrently not only to discriminate A. iwayomogi from other Artemisia plants, but also to identify Artemisia plants using a single PCR process.

Key words

Artemisia plants Random amplified polymorphic DNA Sequence-characterized amplified region marker Multiplex PCR 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Biological sciencesKonkuk UniversitySeoulRepublic of Korea
  2. 2.Department of Biological sciencesKonkuk UniversitySeoulSouth Korea

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