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Development of Sequence Characterized Amplified Region from Random Amplified Polymorphic DNA Amplicons

  • Kalpana JoshiEmail author
  • Preeti Chavan
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 862)

Abstract

Among the PCR-based markers that are most widely used in molecular genetic studies, SCARs (sequence characterized amplified regions) have the advantage of being less sensitive to the conditions of a standard PCR due to its primer size when compared to RAPD (random amplified polymorphic DNA) and hence are more specific and reproducible. Moreover, SCARs require no radioactive isotopes and detect only a single locus. Here, we describe the development of SCAR from RAPD amplicons.

Key words

SCAR Polymorphisms DNA marker Genotyping 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of BiotechnologySinhgad College of EngineeringPuneIndia
  2. 2.Interactive Research School for Health AffairsBharati Vidyapeeth UniversityPuneIndia

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