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The Sleeping Beauty Transposon Toolbox

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Book cover Mobile Genetic Elements

Part of the book series: Methods in Molecular Biology ((MIMB,volume 859))

Abstract

The mobility of class II transposable elements (DNA transposons) can be experimentally controlled by separating the two functional components of the transposon: the terminal inverted repeat sequences that flank a gene of interest to be mobilized and the transposase protein that can be conditionally supplied to drive the transposition reaction. Thus, a DNA molecule of interest (e.g., a fluorescent marker, an shRNA expression cassette, a mutagenic gene trap or a therapeutic gene construct) cloned between the inverted repeat sequences of a transposon-based vector can be stably integrated into the genome in a regulated and highly efficient manner. Sleeping Beauty (SB) was the first transposon ever shown capable of gene transfer in vertebrate cells, and recent results confirm that SB supports a full spectrum of genetic engineering in vertebrate species, including transgenesis, insertional mutagenesis, and therapeutic somatic gene, transfer both ex vivo and in vivo. This methodological paradigm opened up a number of avenues for genome manipulations for basic and applied research. This review highlights the state-of-the-art in SB transposon technology in diverse genetic applications with special emphasis on the transposon as well as transposase vectors currently available in the SB transposon toolbox.

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Acknowledgments

Work in the authors’ laboratories was supported by EU FP6 (INTHER) and EU FP7 (PERSIST and InduStem), grants from the Deutsche Forschungsgemeinschaft SPP1230 “Mechanisms of gene vector entry and persistence,” and from the Bundesministerium für Bildung und Forschung (NGFN-2, NGFNplus, iGene, InTherGD, and ReGene).

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Authors and Affiliations

  1. Max Delbruck Center for Molecular Medicine, Berlin, Germany

    Ismahen Ammar, Zsuzsanna Izsvák & Zoltán Ivics

  2. University of Debrecen, Debrecen, Hungary

    Zsuzsanna Izsvák & Zoltán Ivics

Authors
  1. Ismahen Ammar
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  2. Zsuzsanna Izsvák
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  3. Zoltán Ivics
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Corresponding author

Correspondence to Zoltán Ivics .

Editor information

Editors and Affiliations

  1. , Physiologie de la Reproduction, UMR INRA-CNRS 6175, n/a, Nouzilly Cedex, 37380, France

    Yves Bigot

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Ammar, I., Izsvák, Z., Ivics, Z. (2012). The Sleeping Beauty Transposon Toolbox. In: Bigot, Y. (eds) Mobile Genetic Elements. Methods in Molecular Biology, vol 859. Humana Press. https://doi.org/10.1007/978-1-61779-603-6_13

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  • DOI: https://doi.org/10.1007/978-1-61779-603-6_13

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-602-9

  • Online ISBN: 978-1-61779-603-6

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