Abstract
DNA barcoding refers to the technique of sequencing a short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene, the “DNA barcode,” from a taxonomically unknown specimen and performing comparisons with a reference library of barcodes of known species origin to establish a species-level identification. The library barcodes gain their value due to an intimate association—through the vouchered specimens from where they came—with other data; particularly Linnaean names, collection localities, and morphology in the form of digital images. Consequently, this chapter details means of efficiently obtaining barcodes along two general streams: rapid barcode assembly to populate the library and retrieval of barcodes from highly prized specimens, but also emphasizes organization and collection of the barcode collaterals.
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References
Floyd R, Wilson JJ, Hebert PDN (2009) DNA barcodes and insect biodiversity. In: Footit RG, Adler PH (eds) Insect biodiversity: science and society. Blackwell Publishing, Oxford, pp 417–431
Ratnasingham S, Hebert PDN (2007) BOLD: The barcode of life data system (www.barcodinglife.org). Mol Ecol Notes 7:355–364
Janzen DH, Hajibabaei M, Burns JM et al (2005) Wedding biodiversity inventory of a large and complex Lepidoptera fauna with DNA barcoding. Phil Trans R Soc Lond B 360:1835–1845
Janzen DH, Hallwachs W, Blandin P, Burns JM, Cadiou J-M, Chacon I et al (2009) Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversity. Mol Ecol Res 9:1–25
Xhou X, Robinson JL, Geraci CJ, Parker CR et al (2011) Accelerated construction of a regional DNA-barcode reference library: caddisflies (Trichoptera) in the Great Smoky Mountains National Park. J Nor Amer Benth Soc 30:131–162
iBOL (2010) Barcoding blitz targets Australian Lepidoptera. Barcode Bulletin 1(4):5
Vaglia T, Haxaire J, Kitching IJ et al (2008) Morphology and DNA barcoding reveal three cryptic species within the Xylophanes neoptolemus and loelia species-group (Lepidoptera: Sphingidae). Zootaxa 1923:18–36
Hausmann A, Hebert PDN, Mitchell A et al (2009) Revision of the Australian Oenochroma vinaria Guenée, 1858 species-complex (Lepidoptera, Geometridae, Oenochrominae): DNA barcoding reveals cryptic diversity and assesses status of type specimen without dissection. Zootaxa 2239:1–21
Wilson JJ, Landry JF, Janzen DH et al (2010) Identity of the ailanthus webworm moth, a complex of two species: evidence from DNA barcoding, morphology and ecology. Zookeys 46: 41–60
Dinca˘ V, Zakharov EV, Hebert PDN, Vila R (2011) Complete DNA barcode reference library for a country’s butterfly fauna reveals high performance for temperate Europe. Proc R Soc Lond B 278:347–355
Virgilio M, Backeljau T, Nevado B, de Meyer M (2010) Comparative performances of DNA barcoding across insect orders. BMC Bioinformatics 11:4567–4573
Vogler AP (2006) Will DNA barcoding advance efforts to conserve biodiversity more efficiently than traditional taxonomic methods? Front Ecol Environ 4:270–272
Pons J, Barraclough TG, Gomez-Zurita J et al (2006) Sequence-based species delimitation for the DNA taxonomy of undescribed insects. Sys Biol 55:595–609
Winter WD Jr (2000) Basic techniques for observing and studying moths & butterflies (Memoir No 5). The Lepidopterist’s Society, Cambridge
Hanner R. (2005) Proposed standards for BARCODE records in INSDC (BRIs). http://barcoding.si.edu/PDF/DWG_data_standards-Final.pdf
Regier JC (2008) Protocols, concepts, and reagents for preparing DNA sequencing templates. Version 12/4/08. http://www.umbi.umd.edu/users/jcrlab/PCR_primers.pdf
Porco D, Rougerie R, Deharveng L, Hebert PDN (2010) Coupling non-destructive DNA extraction and voucher retrieval for small soft-bodied Arthropods in a high-throughput context: the example of Collembola. Mol Ecol Res 10: 942–945
Knölke S, Erlacher S, Hausmann A et al (2005) A procedure for combined genitalia extraction and DNA extraction in Lepidoptera. Insect Syst Evol 35:401–409
Ivanova NV, deWaard J, Hebert PDN (2006) An inexpensive, automation-friendly protocol for recovering high-quality DNA. Mol Ecol Notes 6:998–1002
Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotech 3: 294–299
Wilson JJ (2010) Assessing the value of DNA barcodes and other priority gene regions for molecular phylogenetics of Lepidoptera. PLoS ONE 5:e10525
Ivanova N, and Grainger C (2006) Protocols: Sequencing. Canadian Centre for DNA Barcoding CCDB Protocols. http://www.dnabarcoding.ca
Shokralla S, Singer GAC, Hajibabaei M (2010) Direct PCR amplification and sequencing of specimens’ DNA from preservative ethanol. BioTechniques 48:232–234
Hebert PDN, Penton EH, Burns J, Janzen DH, Hallwachs W (2004) Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly, Astraptes fulgerator. Proc Nat Acad Sci USA 101: 14812–14817
Hajibabaei M, Janzen DH, Burns JM, Hallwachs W, Hebert PDN (2006) DNA barcodes distinguish species of tropical Lepidoptera. Proc Nat Acad Sci USA 103:968–971
Messing J (1983) New M13 vectors for cloning. Meth Enzymol 101:20–78
Acknowledgment
Heather Braid compiled the “Barcoding in the Hanner Lab” protocols (http://barcoding.wikia.com/wiki/Barcoding_in_the_Hanner_Lab_Wiki), which greatly aided with the structuring and content of this chapter, and also provided Fig. 1.
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Wilson, J.J. (2012). DNA Barcodes for Insects. In: Kress, W., Erickson, D. (eds) DNA Barcodes. Methods in Molecular Biology, vol 858. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-591-6_3
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DOI: https://doi.org/10.1007/978-1-61779-591-6_3
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