Introduction to Animal DNA Barcoding Protocols
Procedures and protocols common to many DNA barcoding projects are summarized. Planning for any project should emphasize front-end procedures, especially the “genetic lockdown” of collected materials for downstream genetic procedures. Steps further into the DNA barcoding process chain, such as sequencing, data processing, and other back-end functions vary slightly, if at all, among projects and are presented elsewhere in the volume. Point-of-collection sample and tissue handling and data/metadata handling are stressed. Specific predictions of the future workflows and mechanics of DNA barcoding are difficult, so focus is on that which most or all future methods and technologies will surely share.
Key wordsDNA barcoding Animals ATBI Genetic preservation
All of us work with teams of taxonomists, field assistants, lab technicians, and colleagues in our own institution and departments as well as at numerous other institutions around the globe—this chapter represents what those people have taught us and learned with us over the years and they share in the effort and credit but are too many to name.
- 3.Baldwin CC, Mounts JH, Smith DG, Weigt LA (2008) Genetic identification and color descriptions of early life-history stages of Belizean Phaeoptyx and Astrapogon (Teleostei: Apogonidae) with comments on identification of adult Phaeoptyx. Zootaxa 2008:1–22Google Scholar
- 4.Baldwin CC, Weigt LA, Smith DG, Mounts JH (2009) Reconciling genetic lineages with species in Western Atlantic Coryphopterus (Teleostei: Gobiidae). Smithson Contrib Mar Sci 38:111–138Google Scholar
- 5.Baldwin CC, CI Castillo, LA Weigt, BC Victor. (2011) Seven new species within western Atlantic Starksia atlantica, S. lepicoelia, and S. sluiteri (Teleostei: Labrisomidae), with comments on congruence of DNA barcodes and species. Zookeys 79:21–72Google Scholar
- 6.Tornabene L, Baldwin C, Weigt LA, Pezold F (2010) Exploring the diversity of Western Atlantic Bathygobius (Teleostei: Gobiidae) with cytochrome c oxidase-I, with descriptions of two new species. Aqua 16:141–170Google Scholar
- 10.Eymann J, Degreef J, Hauser C, Monje JC, Samyn Y, VandenSpiegel D (2010) Manual on field recording techniques and protocols for all taxa biodiversity inventories. ABC Taxa 8:653Google Scholar
- 12.Templado J, Paulay G, Gittenberger A, Meyer C (2010) Sampling the marine realm. Chapter 11 In: Eymann J, Degreef J, Hauser C et al (eds) Manual on field recording techniques and protocols for all taxa biodiversity inventories. vol 8 ABC Taxa (Belgium), pp 653Google Scholar
- 13.Simon C, Frati F, Beckenbach A et al (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann Ento Soc Am 87:651–701Google Scholar