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Introduction to Animal DNA Barcoding Protocols

  • Lee A. WeigtEmail author
  • Amy C. Driskell
  • Andrea Ormos
  • Christopher Meyer
  • Allen Collins
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 858)

Abstract

Procedures and protocols common to many DNA barcoding projects are summarized. Planning for any project should emphasize front-end procedures, especially the “genetic lockdown” of collected materials for downstream genetic procedures. Steps further into the DNA barcoding process chain, such as sequencing, data processing, and other back-end functions vary slightly, if at all, among projects and are presented elsewhere in the volume. Point-of-collection sample and tissue handling and data/metadata handling are stressed. Specific predictions of the future workflows and mechanics of DNA barcoding are difficult, so focus is on that which most or all future methods and technologies will surely share.

Key words

DNA barcoding Animals ATBI Genetic preservation 

Notes

Acknowledgments

All of us work with teams of taxonomists, field assistants, lab technicians, and colleagues in our own institution and departments as well as at numerous other institutions around the globe—this chapter represents what those people have taught us and learned with us over the years and they share in the effort and credit but are too many to name.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Lee A. Weigt
    • 1
    Email author
  • Amy C. Driskell
    • 1
  • Andrea Ormos
    • 1
  • Christopher Meyer
    • 2
  • Allen Collins
    • 3
  1. 1.Laboratories of Analytical BiologySmithsonian Institution, NMNHSuitlandUSA
  2. 2.Department of Invertebrate Zoology, MRC-163National Museum of Natural History, Smithsonian InstitutionWashingtonUSA
  3. 3.Department of Invertebrate ZoologySmithsonian Institution, NMNHWashingtonUSA

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