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Introduction to Animal DNA Barcoding Protocols

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Book cover DNA Barcodes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 858))

Abstract

Procedures and protocols common to many DNA barcoding projects are summarized. Planning for any project should emphasize front-end procedures, especially the “genetic lockdown” of collected materials for downstream genetic procedures. Steps further into the DNA barcoding process chain, such as sequencing, data processing, and other back-end functions vary slightly, if at all, among projects and are presented elsewhere in the volume. Point-of-collection sample and tissue handling and data/metadata handling are stressed. Specific predictions of the future workflows and mechanics of DNA barcoding are difficult, so focus is on that which most or all future methods and technologies will surely share.

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References

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Acknowledgments

All of us work with teams of taxonomists, field assistants, lab technicians, and colleagues in our own institution and departments as well as at numerous other institutions around the globe—this chapter represents what those people have taught us and learned with us over the years and they share in the effort and credit but are too many to name.

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Authors and Affiliations

  1. Laboratories of Analytical Biology, Smithsonian Institution, NMNH, Suitland, MD, USA

    Lee A. Weigt, Amy C. Driskell & Andrea Ormos

  2. Department of Invertebrate Zoology, MRC-163, National Museum of Natural History, Smithsonian Institution, Washington, DC, USA

    Christopher Meyer

  3. Department of Invertebrate Zoology, Smithsonian Institution, NMNH, Washington, DC, USA

    Allen Collins

Authors
  1. Lee A. Weigt
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  2. Amy C. Driskell
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  3. Andrea Ormos
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  4. Christopher Meyer
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  5. Allen Collins
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Corresponding author

Correspondence to Lee A. Weigt .

Editor information

Editors and Affiliations

  1. National Museum of Natural History, Dept. Botany, Smithsonian Institution, 10th St. & Constitution Ave. NW.,, Washington, 20560-0166, District of Columbia, USA

    W. John Kress

  2. National Museum of Natural History, Dept. Botany, Smithsonian Institution, 10th St. & Constitution Ave. NW.,, Washington, 20560-0166, District of Columbia, USA

    David L. Erickson

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© 2012 Springer Science+Business Media, LLC

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Weigt, L.A., Driskell, A.C., Ormos, A., Meyer, C., Collins, A. (2012). Introduction to Animal DNA Barcoding Protocols. In: Kress, W., Erickson, D. (eds) DNA Barcodes. Methods in Molecular Biology, vol 858. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-591-6_2

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  • DOI: https://doi.org/10.1007/978-1-61779-591-6_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-590-9

  • Online ISBN: 978-1-61779-591-6

  • eBook Packages: Springer Protocols

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