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Multifluorescence 2D Gel Imaging and Image Analysis

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Difference Gel Electrophoresis (DIGE)

Part of the book series: Methods in Molecular Biology ((MIMB,volume 854))

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Abstract

Although image acquisition and analysis are crucial steps within the multifluorescence two-dimensional gel electrophoresis workflow, some basics are frequently not carried out with the necessary diligence. This chapter should help to prevent easily avoidable failures during imaging and image preparation for comparative protein analysis.

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References

  1. GE Healthcare Handbook: Fluorescence imaging: Principles and Methods, GE Healthcare Life Science (2002) 63-0035–28.

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Author information

Authors and Affiliations

  1. Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, Düsseldorf, Germany

    Ingo Vormbrock, Sonja Hartwig & Stefan Lehr

Authors
  1. Ingo Vormbrock
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  2. Sonja Hartwig
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  3. Stefan Lehr
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Corresponding author

Correspondence to Stefan Lehr .

Editor information

Editors and Affiliations

  1. BioCentre, University of Reading, Reading, United Kingdom

    Rainer Cramer

  2. SERVA Electrophoresis GmbH, Carl-Benz-Strasse 7, Heidelberg, 69115, Germany

    Reiner Westermeier

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© 2012 Springer Science+Business Media, LLC

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Vormbrock, I., Hartwig, S., Lehr, S. (2012). Multifluorescence 2D Gel Imaging and Image Analysis. In: Cramer, R., Westermeier, R. (eds) Difference Gel Electrophoresis (DIGE). Methods in Molecular Biology, vol 854. Humana Press. https://doi.org/10.1007/978-1-61779-573-2_3

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  • DOI: https://doi.org/10.1007/978-1-61779-573-2_3

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-572-5

  • Online ISBN: 978-1-61779-573-2

  • eBook Packages: Springer Protocols

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