Abstract
Two-dimensional difference gel electrophoresis is an invaluable technique for the analysis of plant proteomes. However, preparation of protein fractions from plant tissues is challenging due to the special features of plant cells: a robust cell wall, large vacuoles which often contain high concentrations of organic acids and a broad range of secondary metabolites like phenolic compounds and pigments. Therefore, protein preparation for difference gel electrophoresis (DIGE) analyses has to be adapted. Here, we describe both a phenolic protein extraction method for plant tissues and an adapted protocol for DIGE labeling of the generated fractions.
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Acknowledgments
The authors would like to thank Jennifer Klodmann, Institute for Plant Genetics, Leibniz Universität Hannover, for critically reading the manuscript.
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Rode, C., Winkelmann, T., Braun, HP., Colditz, F. (2012). DIGE Analysis of Plant Tissue Proteomes Using a Phenolic Protein Extraction Method. In: Cramer, R., Westermeier, R. (eds) Difference Gel Electrophoresis (DIGE). Methods in Molecular Biology, vol 854. Humana Press. https://doi.org/10.1007/978-1-61779-573-2_23
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DOI: https://doi.org/10.1007/978-1-61779-573-2_23
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