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Difference Gel Electrophoresis (DIGE) pp 287–297Cite as

Method for Protein Subfractionation of Cardiovascular Tissues Before DIGE Analysis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 854))

Abstract

Difference gel electrophoresis (DIGE) (Electrophoresis 18, 2071–2077, 1997, 1) is widely used in cardiovascular research. However, the dynamic range limitations stemming from contaminating plasma proteins and highly abundant extracellular matrix components can make cardiovascular tissues difficult to analyze. Here we describe a novel methodology for biochemical subfractionation of cardiovascular tissues before DIGE analysis.

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Acknowledgements

M.M. is a senior fellow of the British Heart Foundation.

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Authors and Affiliations

  1. Cardiovascular Division, King’s College London, London, UK

    Athanasios Didangelos, Xiaoke Yin & Manuel Mayr

Authors
  1. Athanasios Didangelos
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  2. Xiaoke Yin
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  3. Manuel Mayr
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Corresponding author

Correspondence to Manuel Mayr .

Editor information

Editors and Affiliations

  1. BioCentre, University of Reading, Reading, United Kingdom

    Rainer Cramer

  2. SERVA Electrophoresis GmbH, Carl-Benz-Strasse 7, Heidelberg, 69115, Germany

    Reiner Westermeier

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Didangelos, A., Yin, X., Mayr, M. (2012). Method for Protein Subfractionation of Cardiovascular Tissues Before DIGE Analysis. In: Cramer, R., Westermeier, R. (eds) Difference Gel Electrophoresis (DIGE). Methods in Molecular Biology, vol 854. Humana Press. https://doi.org/10.1007/978-1-61779-573-2_20

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  • DOI: https://doi.org/10.1007/978-1-61779-573-2_20

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-572-5

  • Online ISBN: 978-1-61779-573-2

  • eBook Packages: Springer Protocols

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