Abstract
Difference gel electrophoresis (DIGE) (Electrophoresis 18, 2071–2077, 1997, 1) is widely used in cardiovascular research. However, the dynamic range limitations stemming from contaminating plasma proteins and highly abundant extracellular matrix components can make cardiovascular tissues difficult to analyze. Here we describe a novel methodology for biochemical subfractionation of cardiovascular tissues before DIGE analysis.
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M.M. is a senior fellow of the British Heart Foundation.
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Didangelos, A., Yin, X., Mayr, M. (2012). Method for Protein Subfractionation of Cardiovascular Tissues Before DIGE Analysis. In: Cramer, R., Westermeier, R. (eds) Difference Gel Electrophoresis (DIGE). Methods in Molecular Biology, vol 854. Humana Press. https://doi.org/10.1007/978-1-61779-573-2_20
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DOI: https://doi.org/10.1007/978-1-61779-573-2_20
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