Abstract
De novo gene synthesis allows the creation of custom DNA molecules without the typical constraints of traditional cloning assembly: scars, restriction site incompatibility, and the quest to find all the desired parts to name a few. Moreover, with the help of computer-assisted design, the perfect DNA molecule can be created along with its matching sequence ready to download. The challenge is to build the physical DNA molecules that have been designed with the software. Although there are several DNA assembly methods, this section presents and describes a method using the polymerase chain assembly (PCA).
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References
Czar MJ, Anderson JC, Bader JS and Peccoud J. (2009) Gene synthesis demystified. Trends Biotechnol 27:63–72.
Xiong AS, Peng RH, Zhuang J, Gao F, Li Y, Cheng Z M and Yao, QH (2008) Chemical gene synthesis: strategies, softwares, error corrections, and applications. FEMS Microbiol 32:522–540.
Cello J, Paul AV and Wimmer E (2002) Chemical Synthesis of Poliovirus cDNA: Generation of Infectious Virus in the Absence of Natural Template. Science 297:1016–1018.
Dymond J, Scheifele L, Richardson S, Lee P, Chandrasegaran S, Bader J and Boeke JD (2009) Teaching Synthetic Biology, Bioinfor-matics, and Engineering to Undergraduates: The Interdisciplinary Build-a-Genome Course. Genetics 18:13–21.
Richardson SM, Wheelan SJ, Yarrington, RM and Boeke, JD (2006) GeneDesign: rapid, automated design of multikilobase synthetic genes. Genome Res 16:550–6.
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© 2012 Springer Sceince+Business Media, LLC
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Marchand, J.A., Peccoud, J. (2012). Building Block Synthesis Using the Polymerase Chain Assembly Method. In: Peccoud, J. (eds) Gene Synthesis. Methods in Molecular Biology, vol 852. Humana Press. https://doi.org/10.1007/978-1-61779-564-0_1
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DOI: https://doi.org/10.1007/978-1-61779-564-0_1
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Online ISBN: 978-1-61779-564-0
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