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A One-Time Inducible Transposon to Create Knockout Mutants in Rice

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Book cover Transgenic Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 847))

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Abstract

Use of a transposon is an efficient tagging tool for exploring the function of the gene it inserts into or is adjacent to. A few modifications have been applied to the native Ac transposon to allow it to transpose efficiently or spontaneously and stop quickly thereafter. Furthermore, locating the transposon between a constitutive plant promoter and a reporter gene, such as the firefly luciferase gene, allows for nondestructively detecting excision events in vivo. This chapter describes a detailed protocol for one-time inducible transposon tagging of rice cells and their subsequent screening and regeneration into mutant lines.

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Author information

Authors and Affiliations

  1. Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China

    Yuh-Chyang Charng

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  1. Yuh-Chyang Charng
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Correspondence to Yuh-Chyang Charng .

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Editors and Affiliations

  1. School of Biological Sciences, University of Reading, Reading, Berkshire, RG6 6AS, United Kingdom

    Jim M. Dunwell

  2. Dept. of Agricultural Botany, School of Biological Sciences, University of Reading, Reading, United Kingdom

    Andy C. Wetten

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Charng, YC. (2012). A One-Time Inducible Transposon to Create Knockout Mutants in Rice. In: Dunwell, J., Wetten, A. (eds) Transgenic Plants. Methods in Molecular Biology, vol 847. Humana Press. https://doi.org/10.1007/978-1-61779-558-9_29

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  • DOI: https://doi.org/10.1007/978-1-61779-558-9_29

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-557-2

  • Online ISBN: 978-1-61779-558-9

  • eBook Packages: Springer Protocols

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