Abstract
The establishment of traits that result from the concerted expression of complementing transgene fragments is a feasible tool for trait control or gene flow control in plants. This chapter describes the methodology for producing herbicide-resistant and pollen-sterile wheat plants by the intein-mediated assembly of inactive precursor protein fragments (protein trans-splicing). We suggest the design of intein-containing vectors for split-transgene expression. We describe transient plant assays that can be used to analyse the functionality of the system and describe the transformation of wheat plants using a split selection marker.
We hope that this chapter will be a helpful guideline for researchers who are interested in applying similar split-gene approaches in wheat or other monocotyledonous crops.
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Acknowledgements
The authors sincerely wish to thank Angelika Gils for her critical reading of the manuscript.
The research was funded by the Bundesministerium für Bildung und Forschung (BMBF, GABI-FUTURE grant 0315043A) at the Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Gatersleben, and in cooperation with the Nordsaat GmbH, Böhnshausen, Germany.
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Gils, M., Rubtsova, M., Kempe, K. (2012). Split-Transgene Expression in Wheat. In: Dunwell, J., Wetten, A. (eds) Transgenic Plants. Methods in Molecular Biology, vol 847. Humana Press. https://doi.org/10.1007/978-1-61779-558-9_11
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DOI: https://doi.org/10.1007/978-1-61779-558-9_11
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