Abstract
Hammerhead ribozymes are small catalytic RNA motifs ubiquitously present in a large variety of genomes. The reactions catalyzed by these motifs are both their self-scission and the reverse ligation reaction. Here, we describe methods for the generation of DNA templates for the subsequent in vitro transcription of hammerhead ribozymes. This is followed by a description of the preparation of suitable RNA molecules for both reaction types, and their kinetic analysis.
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Kalweit, A., Przybilski, R., Seehafer, C., de la Peña, M., Hammann, C. (2012). Characterization of Hammerhead Ribozyme Reactions. In: Hartig, J. (eds) Ribozymes. Methods in Molecular Biology, vol 848. Humana Press. https://doi.org/10.1007/978-1-61779-545-9_2
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DOI: https://doi.org/10.1007/978-1-61779-545-9_2
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