Abstract
Cultured chromaffin cells have been used for almost 40 years in the study of different cell functions using biochemical, electrophysiological, pharmacological, and toxicological approaches. Chromaffin cells are essentially secretory cells that are used to model sympathetic neurons or neuroendocrine cells. In this chapter, we describe the most common methods currently used to isolate and culture chromaffin cells from the animals used most commonly: cows, rats, and mice. We also provide some advice on the use of these cells in the laboratory.
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Acknowledgments
We are indebted to the contributions of our predecessors working in the field of cell culture. This work was supported by the Spanish Ministry of Science and Innovation (MICINN, Grants # BFU BFU2007-64963, CSD2008-00005 and BFU2010-15822) and the Canary Islands Government (GC, Grants # C2008/01000239). N.D. is a recipient of an FPU scholarship from MICINN, M.R. received a grant from CajaCanarias, and J.D.M. is the holder of a “Ramón y Cajal” contract from the MICINN.
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Domínguez, N., Rodríguez, M., Machado, J.D., Borges, R. (2012). Preparation and Culture of Adrenal Chromaffin Cells. In: Skaper, S. (eds) Neurotrophic Factors. Methods in Molecular Biology, vol 846. Humana Press. https://doi.org/10.1007/978-1-61779-536-7_20
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DOI: https://doi.org/10.1007/978-1-61779-536-7_20
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