Abstract
Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once they become mature unless given a growth-permissive environment, for example, a peripheral nerve graft. Retinal ganglion cells (RGCs) of the optic nerve represent a highly useful cell model for the study of neurotrophic factor responsiveness, although the presence of nonneuronal cells in the retina makes it difficult to interpret the direct effects of tested factors on RGCs. Cultures of purified RGCs thus represent an excellent tool for the study of optic nerve cell trophic responsiveness, in terms of both survival and axonal regeneration.
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References
David S, and Aguayo AJ (1981) Axonal elongation into peripheral nervous system “bridges” after central nervous system injury in adult rats. Science 214, 931–933
Gervasi NM, Kwok JC, and Fawcett JW (2008) Role of extracellular factors in axon regeneration in the CNS: implications for therapy. Regen Med 3, 907–923
Mansour-Robaey S, Clarke DB, Wang YC, Bray GM, and Aguayo AJ (1994) Effects of ocular injury and administration of brain-derived neurotrophic factor on survival and regrowth of axotomized retinal ganglion cells. Proc Natl Acad Sci USA 91, 1,632–1,636
Mey J, and Thanos S (1993) Intravitreal injections of neurotrophic factors support the survival of axotomized retinal ganglion cells in adult rats in vivo. Brain Res 602, 304–317
Peinado-Ramon P, Salvador M, Villegas-Perez MP, and Vidal-Sanz M (1996) Effects of axotomy and intraocular administration of NT-4, NT-3, and brain-derived neurotrophic factor on the survival of adult rat retinal ganglion cells. A quantitative in vivo study. Invest Ophthalmol Vis Sci 37, 489–500
Clarke DB, Bray GM, and Aguayo AJ (1998) Prolonged administration of NT-4/5 fails to rescue most axotomized retinal ganglion cells in adult rats. Vision Res 38, 1,517–1,524
Carmignoto G, Maffei L, Candeo P, Canella R, and Comelli C (1989) Effect of NGF on the survival of rat retinal ganglion cells following optic nerve section. J Neurosci 9, 1,263–1,272
Lenzi L, Coassin M, Lambiase A, Bonini S, Amendola T, and Aloe L (2005) Effect of exogenous administration of nerve growth factor in the retina of rats with inherited retinitis pigmentosa. Vision Res 45, 1,491–1,500
Cui Q, Yip HK, Zhao RC, So KF, and Harvey AR (2003) Intraocular elevation of cyclic AMP potentiates ciliary neurotrophic factor-induced regeneration of adult rat retinal ganglion cell axons. Mol Cell Neurosci 22, 49–61
Leaver SG, Cui Q, Plant GW, Arulpragasam A, Hisheh S, Verhaagen J, et al (2006) AAV-mediated expression of CNTF promotes long-term survival and regeneration of adult rat retinal ganglion cells. Gene Ther 13, 1,328–1,341
Rios-Munoz W, Soto I, Duprey-Diaz MV, Blagburn J, and Blanco RE (2005) Fibroblast growth factor 2 applied to the optic nerve after axotomy increases Bcl-2 and decreases Bax in ganglion cells by activating the extracellular signal-regulated kinase signaling pathway. J Neurochem 93, 1,422–1,433
Unoki K, and LaVail MM (1994) Protection of the rat retina from ischemic injury by brain-derived neurotrophic factor, ciliary neurotrophic factor, and basic fibroblast growth factor. Invest Ophthalmol Vis Sci 35, 907–915
Ma CHM, and Taylor JSH (2010) Trophic responsiveness of purified postnatal and adult rat retinal ganglion cells. Cell Tissue Res 339, 297–310
Barres BA, Silverstein BE, Corey DP, and Chun LL (1988) Immunological, morphological, and electrophysiological variation among retinal ganglion cells purified by panning. Neuron 1, 791–803
Zhang XM, Li Liu DT, Chiang SW, Choy KW, Pang CP, Lam DS, et al (2010) Immunopanning purification and long-term culture of human retinal ganglion cells. Mol Vis 16, 2,867–2,872
Bader DR, MacLeish PR, and Schwartz EA (1978) Responses to light of solitary rod photoreceptors isolated from tiger salamander retina. Proc Natl Acad Sci USA 75, 3,507–3,511
Huettner JE, and Baughman RW (1986) Primary culture of identified neurons from the visual cortex of postnatal rats. J Neurosci 6, 3,044–3,060
Wysocki LJ, and Sato VL (1978) “Panning” for lymphocytes: a method for cell selection. Proc Natl Acad Sci USA 75, 2,844–2,848
Brewer GJ, Torricelli JR, Evege EK, and Price PJ (1993) Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination. J Neurosci Res 35, 567–576
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65, 55–63
Manthorpe M, Fagnani R, Skaper SD, and Varon S (1986) An automated colorimetric microassay for neuronotrophic factors. Dev Brain Res 25, 191–198
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Skaper, S.D. (2012). Rodent Retinal Ganglion Cell Cultures. In: Skaper, S. (eds) Neurotrophic Factors. Methods in Molecular Biology, vol 846. Humana Press. https://doi.org/10.1007/978-1-61779-536-7_11
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DOI: https://doi.org/10.1007/978-1-61779-536-7_11
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