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Functional Circuitry Analysis in Rodents Using Neurotoxins/Immunotoxins

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Controlled Genetic Manipulations

Part of the book series: Neuromethods ((NM,volume 65))

Abstract

Immunotoxin cell targeting is a transgenic animal technology used to eliminate specific cell types from a complex neural circuitry by using cytotoxic activity of recombinant immunotoxins, which are composed of an antibody variable region fused to bacterial toxin fragments. This technology provides a useful approach for studying the neural circuitry mechanism underlying a variety of brain functions. The present chapter provides a detailed experimental strategy for immunotoxin cell targeting and its application to neural circuitry analysis, in particular focusing on the basal ganglia circuitry, which is implicated in the control of motor functions.

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Authors and Affiliations

  1. Department of Molecular Genetics, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima, Japan

    Kazuto Kobayashi, Kana Okada & Nobuyuki Kai

Authors
  1. Kazuto Kobayashi
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  2. Kana Okada
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  3. Nobuyuki Kai
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Corresponding author

Correspondence to Kazuto Kobayashi .

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Editors and Affiliations

  1. National Institute of Mental Health, National Institutes of Health, Convent Drive 35, Bethesda, 20892, Maryland, USA

    Alexei Morozov

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Kobayashi, K., Okada, K., Kai, N. (2012). Functional Circuitry Analysis in Rodents Using Neurotoxins/Immunotoxins. In: Morozov, A. (eds) Controlled Genetic Manipulations. Neuromethods, vol 65. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-533-6_10

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  • DOI: https://doi.org/10.1007/978-1-61779-533-6_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-532-9

  • Online ISBN: 978-1-61779-533-6

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