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Cardiovascular Development pp 69–78Cite as

Whole-Mount Confocal Microscopy for Vascular Branching Morphogenesis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 843))

Abstract

We introduce a whole-mount immunohistochemistry method for analyzing intricate vascular network formation in mouse embryonic tissues. Laser scanning confocal microscopy with multiple labeling allows for robust imaging of blood and lymphatic vessel branching morphogenesis with excellent resolution.

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References

  1. Mukouyama, Y.S., Shin, D., Britsch, S., Taniguchi, M., Anderson, D.J. (2002) Sensory nerves determine the pattern of arterial differentiation and blood vessel branching in the skin. Cell. 109, 693–705.

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  2. Hibbs, A.R. (2004) Confocal Microscopy for Biologist. Springer

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  3. Wang, H.U., Chen, Z.F., Anderson, D.J. (1998) Molecular distinction and angiogenic interaction between embryonic arteries and veins revealed by ephrin-B2 and its receptor Eph-B4. Cell. 93, 741–53.

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Acknowledgments

Work supported by Intramural Research Program of National Institutes of Health. We thank Dr. Wenling Li for critical comments on this manuscript. We are also grateful to Heesuk Zang, John Hatch, and Izumi Onitsuka for their contributions to this whole-mount immunohistochemistry method.

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Authors and Affiliations

  1. Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA

    Yoh-suke Mukouyama, Jennifer James, Joseph Nam & Yutaka Uchida

Authors
  1. Yoh-suke Mukouyama
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  2. Jennifer James
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  3. Joseph Nam
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  4. Yutaka Uchida
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Corresponding author

Correspondence to Yoh-suke Mukouyama .

Editor information

Editors and Affiliations

  1. College of Medicine, Dept. Systems Biology &, Texas A&M Health Science Center, SW. HK Dodgen Loop 702, Temple, 76504-7105, Texas, USA

    Xu Peng

  2. School of Veterinary Medicine, Department of Molecular Medicine, Cornell University, Ithaca, 14853, New York, USA

    Marc Antonyak

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© 2012 Springer Science+Business Media, LLC

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Mukouyama, Ys., James, J., Nam, J., Uchida, Y. (2012). Whole-Mount Confocal Microscopy for Vascular Branching Morphogenesis. In: Peng, X., Antonyak, M. (eds) Cardiovascular Development. Methods in Molecular Biology, vol 843. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-523-7_7

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  • DOI: https://doi.org/10.1007/978-1-61779-523-7_7

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-522-0

  • Online ISBN: 978-1-61779-523-7

  • eBook Packages: Springer Protocols

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