Abstract
Cardiovascular development is a precisely coordinated process at multilevels. It involves cross-talking among numerous signaling transduction pathways to ensure proper cell polarity, migration, proliferation, differentiation, and programmed death. Here, genome-wide RNA interference screens in Drosophila cells are introduced as novel approaches to discover potential regulators, with special emphases on (1) cell growth and viability, and (2) receptor tyrosine kinase and extracellular-signal-regulated kinase signaling pathway.
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Acknowledgments
The authors would like to thank Professor Norbert Perrimon, and staff of the Drosophila RNAi Screening Center at Harvard Medical School.
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Zhang, S., Zheng, H. (2012). The Application of Genome-Wide RNAi Screens in Exploring Varieties of Signaling Transduction Pathways. In: Peng, X., Antonyak, M. (eds) Cardiovascular Development. Methods in Molecular Biology, vol 843. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-523-7_21
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DOI: https://doi.org/10.1007/978-1-61779-523-7_21
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