Abstract
Apoptosis is the process of programmed cell death that has been identified in the development of heart. It is likely to be regulated by survival and death signals that are also present in many other tissues. To understand cardiomyocyte apoptosis in normal and abnormal development of heart, the cell death detection techniques were applied to various researches. These methods include morphological, histological, or molecular assays based on recent advances in our understanding of the molecular mechanism of cell death, including: (1) terminal deoxynucleotidyl transferase mediated dUTP nick end-labeling of fragmented nuclei, (2) cardiovascular molecular imaging of apoptosis using Annexin V, and (3) immunohistochemical detection of activated caspases.
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References
Poelmann, R.E., and Groot, A.C.G.-d. (2005) Apoptosis as an instrument in cardiovascular development. Birth Defects Research Part C: Embryo Today: Reviews 75, 305–313.
Fisher, S.A., Langille, B.L., and Srivastava, D. (2000) Apoptosis During Cardiovascular Development. Circ Res 87, 856–864.
G, M., and I, J. (1995) Apoptosis, oncosis, and necrosis. An overview of cell death. Am J Pathol 146(1), 3–15
Kunapuli, S., Rosanio, S., and Schwarz, E.R. (2006) “How Do Cardiomyocytes Die?” Apoptosis and Autophagic Cell Death in Cardiac Myocytes. Journal of Cardiac Failure 12, 381–391.
SJ, Martin., CP, Reutelingsperger., AJ, McGahon., JA, Rader., RC, van Schie., DM, LaFace., and DR, Green. (1995) Early redistribution of plasma membrane phosphatidylserine is a general feature. J Exp Med 182(5), 1545–56
Scarabelli, T.M., Knight, R., Stephanou, A., Townsend, P., Chen-Scarabelli, C., Lawrence, K., Gottlieb, R., Latchman, D., and Narula, J. (2006). Clinical Implications of Apoptosis in Ischemic Myocardium. Current Problems in Cardiology 31, 181–264.
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Qi, D., Fu, M. (2012). Cardiomyocyte Apoptosis in Heart Development: Methods and Protocols. In: Peng, X., Antonyak, M. (eds) Cardiovascular Development. Methods in Molecular Biology, vol 843. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-523-7_18
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DOI: https://doi.org/10.1007/978-1-61779-523-7_18
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-522-0
Online ISBN: 978-1-61779-523-7
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