Abstract
Through directed differentiation of embryonic stem cells, it has been demonstrated that mesodermal lineages in the mammalian heart (smooth muscle, endothelial, and cardiac) develop from a common, multipotent cardiovascular precursor (Dev Biol 265:262–275, 2004; Cell 127:1137–1150, 2006; Dev Cell 11:723–732, 2006). Identification of cardiovascular precursor cells at various stages of lineage commitment has been determined by expression of multiple markers, including the stem cell factor receptor c-kit. Utilizing a bacterial artificial chromosome (BAC) transgenic mouse model in which EGFP expression is placed under control of the c-kit promoter (c-kitBAC-EGFP), work from our laboratory indicates that c-kit expression identifies a multipotent cardiovascular precursor cell population within the early postnatal heart that can be isolated, expanded, and differentiated in vitro into all three cell lineages that specify the heart (Proc Natl Acad Sci U S A 106:1808–1813, 2009).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Martin, C. M., Meeson, A. P., Robertson, S. M., Hawke, T. J., Richardson, J. A., Bates, S., Goetsch, S. C., Gallardo, T. D., and Garry, D. J. (2004) Persistent Expression of the ATP-Binding Cassette Transporter, Abcg2, Identifies Cardiac SP Cells in the Developing and Adult Heart. Developmental Biology. 265, 262–275.
Wu, S. M., Fujiwara, Y., Cibulsky, S. M., Clapham, D. E., Lien, C., Schultheiss, T. M., and Orkin, S. H. (2006) Developmental Origin of a Bipotential Myocardial and Smooth Muscle Cell Precursor in the Mammalian Heart. Cell. 127, 1137–1150.
Kattman, S. J., Huber, T. L., and Keller, G. M. (2006) Multipotent Flk-1+ Cardiovascular Progenitor Cells Give Rise to the Cardiomyocyte, Endothelial, and Vascular Smooth Muscle Lineages. Dev. Cell. 11, 723–732.
Tallini, Y. N., Greene, K. S., Craven, M., Spealman, A., Breitbach, M., Smith, J., Fisher, P. J., Steffey, M., Hesse, M., Doran, R. M., Woods, A., Singh, B., Yen, A., Fleischmann, B. K., and Kotlikoff, M. I. (2009) C-Kit Expression Identifies Cardio-vascular Precursors in the Neonatal Heart. Proc. Natl. Acad. Sci. U. S. A. 106, 1808–1813.
Reber, L., Da Silva, C. A., and Frossard, N. (2006) Stem Cell Factor and its Receptor c-Kit as Targets for Inflammatory Diseases. Eur. J. Pharmacol. 533, 327–340.
Naqvi, N., Li, M., Yahiro, E., Graham, R. M., and Husain, A. (2009) Insights into the Characteristics of Mammalian Cardiomyocyte Terminal Differentiation shown through the Study of Mice with a Dysfunctional c-Kit. Pediatr. Cardiol. 30, 651–658.
Yasuda, H., Galli, S. J., and Geissler, E. N. (1993) Cloning and Functional Analysis of the Mouse c-Kit Promoter. Biochem. Biophys. Res. Commun. 191, 893–901.
Alexeev, V., and Yoon, K. (2006) Distinctive Role of the cKit Receptor Tyrosine Kinase Signaling in Mammalian Melanocytes. J. Invest. Dermatol. 126, 1102–1110.
Beltrami, A. P., Barlucchi, L., Torella, D., Baker, M., Limana, F., Chimenti, S., Kasahara, H., Rota, M., Musso, E., Urbanek, K., Leri, A., Kajstura, J., Nadal-Ginard, B., and Anversa, P. (2003) Adult Cardiac Stem Cells are Multipotent and Support Myocardial Regeneration. Cell. 114, 763–776.
Gude, N., Muraski, J., Rubio, M., Kajstura, J., Schaefer, E., Anversa, P., and Sussman, M. A. (2006) Akt Promotes Increased Cardio-myocyte Cycling and Expansion of the Cardiac Progenitor Cell Population. Circ. Res. 99, 381–388.
Acknowledgments
The authors would like to thank Stanka Semova and Sergei Rudchenko of the Flow Cytometry Facility at the Hospital for Special Surgery in New York, NY and Lavanya Gowri Sayam of the Biomedical Sciences Flow Cytometry Core at Cornell University in Ithaca, NY for their technical input and guidance. The authors would also like to thank their coauthors on the 2009 PNAS manuscript (Ref. (4)), without whom this work would not have been possible. This work was supported by R01GM086736 and R01DK065992.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Craven, M., Kotlikoff, M.I., Nadworny, A.S. (2012). C-kit Expression Identifies Cardiac Precursor Cells in Neonatal Mice. In: Peng, X., Antonyak, M. (eds) Cardiovascular Development. Methods in Molecular Biology, vol 843. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-523-7_17
Download citation
DOI: https://doi.org/10.1007/978-1-61779-523-7_17
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-522-0
Online ISBN: 978-1-61779-523-7
eBook Packages: Springer Protocols