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Structure-Based Drug Discovery pp 179–207Cite as

The Role of Enzymology in a Structure-Based Drug Discovery Program: Bacterial DNA Gyrase

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 841))

Abstract

The capability to accurately, rapidly, and reproducibly determine the affinity of a ligand for a target protein or enzyme is a vital component for a successful structure-based drug design effort. In order to successfully drive a structure-based drug design (SBDD) project forward, multiple distinct assays, each with particular strengths and weaknesses, need to be employed. Using bacterial DNA gyrase as an example, a range of assays are described that will fully support an SBDD program.

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Authors and Affiliations

  1. Trius Therapeutics, San Diego, CA, USA

    Mark L. Cunningham

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  1. Mark L. Cunningham
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Correspondence to Mark L. Cunningham .

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  1. Trius Therapeutics, Nancy Ridge Dr. 6310, San Diego, 92121, California, USA

    Leslie W. Tari

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Cunningham, M.L. (2012). The Role of Enzymology in a Structure-Based Drug Discovery Program: Bacterial DNA Gyrase. In: Tari, L. (eds) Structure-Based Drug Discovery. Methods in Molecular Biology, vol 841. Humana Press. https://doi.org/10.1007/978-1-61779-520-6_8

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  • DOI: https://doi.org/10.1007/978-1-61779-520-6_8

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-519-0

  • Online ISBN: 978-1-61779-520-6

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