Abstract
DNA extracted from keratinous and chitinous materials can be a useful source of genetic information. To effectively liberate the DNA from these materials, buffers containing relatively high levels of DTT, proteinase K, and detergent are recommended, followed by purification using either silica-column or organic methods.
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Acknowledgments
MTPG was supported by the Danish National Science Foundation’s “Skou” grant program.
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© 2012 Springer Science+Business Media, LLC
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Campos, P.F., Gilbert, T.M.P. (2012). DNA Extraction from Keratin and Chitin. In: Shapiro, B., Hofreiter, M. (eds) Ancient DNA. Methods in Molecular Biology, vol 840. Humana Press. https://doi.org/10.1007/978-1-61779-516-9_6
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DOI: https://doi.org/10.1007/978-1-61779-516-9_6
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Publisher Name: Humana Press
Print ISBN: 978-1-61779-515-2
Online ISBN: 978-1-61779-516-9
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