Role of Prickle1 and Prickle2 in Neurite Outgrowth in Murine Neuroblastoma Cells

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 839)

Abstract

Murine Prickle2 but not Prickle1 gene expression was induced in C1300 neuroblastoma cell line during neurite-like process formation induced by all trans-retinoic acid (RA). Overexpression of Prickle1 or Prickle2 in C1300 cells induced striking neurite-like process formation without RA. Prickle1 and Prickle2 associate with Dishevelled1 (Dvl1) and overexpression of Prickle1 or Prickle2 resulted in the reduction of Dvl1 protein in C1300 cells. Overexpression of Dvl1 in C1300 cells prevented the neurite-like process formation induced by Prickle1 or Prickle2 overexpression. Prickle1 and Prickle2 promote neurite-like process formation of C1300 cells via the Dvl1-dependent mechanism.

Key words

Neuroblastoma cell line Mouse cerebellum granule cell neurons Prickle1 Prickle2 Dishevelled1 All trans-retinoic acid 

Notes

Acknowledgments

The authors would like to thank A. Goda and M. Hanazono for skillful technical assistance and H. Takano for discussion. This work was supported in part by Grants-in-Aid from the Ministry of Education, Science, Technology, Sports, and Culture of Japan.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Biomedical Science, Graduate School of MedicineChiba UniversityChiba CityJapan

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