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Examining Planar Cell Polarity in the Mammalian Cochlea

  • Helen May-Simera
  • Matthew W. Kelley
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 839)

Abstract

The mammalian cochlea offers a unique opportunity to study the effects of planar cell polarity signaling during vertebrate development. First, convergence and extension play a role in outgrowth and cellular patterning within the duct, and second, hair cell stereociliary bundles are uniformly oriented towards the lateral edge of the duct. Defects in convergence and extension are manifested as a shortening of the cochlea duct and/or changes in cellular patterning, which can be quantified following dissection from mouse mutants or observed directly using an in vitro outgrowth assay. Changes in stereociliary bundle orientation can be observed and quantitated using either fluorescent tags or scanning electron microscopy (SEM) to visualize individual bundles. The high degree of regularity in many aspects of cochlear anatomy, including cellular patterning and stereociliary bundle orientation, makes it possible to detect subtle changes in the development of PCP in response to either genetic or molecular perturbations.

Key words

Cochlea Hair cells Development Organ of Corti Stereociliary bundle Kinocilium Microtubules Actin Vangl2 Wnt 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Laboratory of Cochlear DevelopmentNational Institute on Deafness and other Communication Disorders, National Institutes of HealthBethesdaUSA

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