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Plant Fungal Pathogens pp 97–105Cite as

Identification of HR-Inducing cDNAs from Plant Pathogens via a Gateway®-Compatible Binary Potato Virus X-Expression Vector

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 835))

Abstract

Identification of pathogen effectors that elicit a hypersensitive response (HR) in resistant plant hosts is essential to study disease resistance. In this method, it is described how to generate a cDNA library, how to transfer the library into a binary PVX-expression vector, and finally how to set up a high-throughput screen for HR-inducing cDNAs from plant pathogens.

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Acknowledgments

H. P. V. E. is supported by a Veni grant of the Research Council for Earth and Life sciences (ALW) of the Netherlands Organization for Scientific Research (NWO). The author thanks Adrian Valli for kindly providing a Gateway®-compatible PVX-vector.

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Authors and Affiliations

  1. Laboratory of Phytopathology, Wageningen University, Wageningen, the Netherlands

    H. Peter van Esse

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  1. H. Peter van Esse
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Correspondence to H. Peter van Esse .

Editor information

Editors and Affiliations

  1. Northern Crops Science Laboratory, USDA-ARS, North 18th Street 1307, Fargo, 58105-5677, North Dakota, USA

    Melvin D. Bolton

  2. Lab. Phytopathology, Wageningen University, Droevendaalsesteeg 1, Wageningen, 6708 PB, Netherlands

    Bart P.H.J. Thomma

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van Esse, H.P. (2012). Identification of HR-Inducing cDNAs from Plant Pathogens via a Gateway®-Compatible Binary Potato Virus X-Expression Vector. In: Bolton, M., Thomma, B. (eds) Plant Fungal Pathogens. Methods in Molecular Biology, vol 835. Humana Press. https://doi.org/10.1007/978-1-61779-501-5_6

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  • DOI: https://doi.org/10.1007/978-1-61779-501-5_6

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-500-8

  • Online ISBN: 978-1-61779-501-5

  • eBook Packages: Springer Protocols

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