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Plant Fungal Pathogens pp 591–602Cite as

Karyotyping Methods for Fungi

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 835))

Abstract

Pulsed field gel electrophoresis enables separation of fungal chromosomes up to several megabases and is a worthwhile tool for fungal karyotyping. The germ tube burst method is a technique to separate fungal chromosomes of any size for chromosome number determination as well as in situ hybridization. Here we provide detailed protocols for both complementary methods that have many applications in fungal biology including chromosome size and chromosome number polymorphisms, and in situ localization of genes on chromosomes.

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Author information

Authors and Affiliations

  1. Seed and Plant Improvement Institute, Karaj, Iran

    Rahim Mehrabi & Mostafa Aghaee

  2. Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands

    Rahim Mehrabi & Pierre J. G. M. de Wit

  3. Department of Biology, Okayama University, Tsushima-naka, Okayama, Japan

    Masatoki Taga

  4. Plant Research International, Wageningen, The Netherlands

    Gert H. J. Kema

Authors
  1. Rahim Mehrabi
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  2. Masatoki Taga
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  3. Mostafa Aghaee
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  4. Pierre J. G. M. de Wit
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  5. Gert H. J. Kema
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Corresponding author

Correspondence to Rahim Mehrabi .

Editor information

Editors and Affiliations

  1. Northern Crops Science Laboratory, USDA-ARS, North 18th Street 1307, Fargo, 58105-5677, North Dakota, USA

    Melvin D. Bolton

  2. Lab. Phytopathology, Wageningen University, Droevendaalsesteeg 1, Wageningen, 6708 PB, Netherlands

    Bart P.H.J. Thomma

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Mehrabi, R., Taga, M., Aghaee, M., de Wit, P.J.G.M., Kema, G.H.J. (2012). Karyotyping Methods for Fungi. In: Bolton, M., Thomma, B. (eds) Plant Fungal Pathogens. Methods in Molecular Biology, vol 835. Humana Press. https://doi.org/10.1007/978-1-61779-501-5_37

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  • DOI: https://doi.org/10.1007/978-1-61779-501-5_37

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-500-8

  • Online ISBN: 978-1-61779-501-5

  • eBook Packages: Springer Protocols

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