Abstract
Nucleotide-binding, leucine-rich repeat (NB-LRR) immune receptors from plants confer resistance to fungal pathogens and many other pathogenic organisms. Their low expression makes it challenging to purify these receptors from plants in sufficient quantities to be able to identify interacting proteins by mass spectrometry. Here we describe a protocol to affinity-purify recombinant NB-LRR immune receptors, fused to the streptavidin-binding peptide tag.
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Acknowledgments
I thank Matthieu Joosten for critical reading of the manuscript. I am grateful to the EU-funded Integrated Project Bioexploit, the Netherlands Organization for Scientific Research (NWO; VENI grant 863.08.018 to WILT) and the Centre for BioSystems Genomics (CBSG), which is part of the Netherlands Genomics Initiative and NWO, for support.
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Tameling, W.I.L. (2012). A One-Step Affinity-Purification Protocol to Purify NB-LRR Immune Receptors from Plants That Mediate Resistance to Fungal Pathogens. In: Bolton, M., Thomma, B. (eds) Plant Fungal Pathogens. Methods in Molecular Biology, vol 835. Humana Press. https://doi.org/10.1007/978-1-61779-501-5_36
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DOI: https://doi.org/10.1007/978-1-61779-501-5_36
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Online ISBN: 978-1-61779-501-5
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