Abstract
Cerebrospinal fluid (CFS) is the most easily accessible component of the human central nervous system and has been successfully used for the analysis of disease-associated molecular imbalances, particularly for extracellular matrix components. Alterations in the presence of the nervous system-associated chondroitin sulfate proteoglycan neurocan had been reported from active multiple sclerosis lesions. Neurocan could be detected as a component of human CFS after enrichment of proteoglycans by anion exchange chromatography from pooled liquor as well as individual 300 μL samples by Western blot. However, a general alteration in neurocan levels in CFS sample with high immunoglobulin content could not be demonstrated. To further reduce the sample size, the development of a PG capturing assay based on polybrene-coated 96-well plates was initiated. This approach could be an interesting alternative option for the analysis of PGs in biological fluid and tissue samples.
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Acknowledgments
I would like to thank Dr. A. Grubb and J. Warenholt for help with the human CSF samples, and the Alfred Österlunds, the H and J Forssmans, the G and J Kocks, and the Crafoords foundations, the Swedish Research Council and Lunds Universities Medical Faculty for support.
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Rauch, U. (2012). Detection of Neurocan in Cerebrospinal Fluid. In: Rédini, F. (eds) Proteoglycans. Methods in Molecular Biology, vol 836. Humana Press. https://doi.org/10.1007/978-1-61779-498-8_6
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DOI: https://doi.org/10.1007/978-1-61779-498-8_6
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