Abstract
In this chapter, we present the methods developed in our lab for the scintigraphic imaging and direct quantitative evaluation of proteoglycan (PG) distribution in vivo. These methods relate to (1) the synthesis and radiolabeling of the NTP 15-5 with 99mTc, (2) preclinical scintigraphic imaging using laboratory animals, and (3) quantitative analysis of scintigraphic images.
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Acknowledgments
In our group, studies related to the in vivo scintigraphy of proteoglycans for the imaging of cartilage and its degenerative and tumoral pathologies are supported by the Regional Fund for Innovation (FRI 2) OSEO, the Contrat de Projet Etat Region (CPER), the Fondation pour la Recherche Médicale (FRM), the Ligue Contre Le Cancer, and the Institut National du Cancer.
Studies aiming at validating 99mTc-NTP 15-5 radiotracer as a candidate for clinical imaging of cartilage pathologies in nuclear medicine are conducted in close collaboration with Cyclopharma Laboratoires.
The authors would like to thank Prof. D. Heymann, Prof. F. Gouin, and Dr. F. Rédini from UMR S957 INSERM from Nantes University for their collaboration and expertise on chondrosarcoma.
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Miot-Noirault, E., Vidal, A., Auzeloux, P., Peyrode, C., Madelmont, JC., Chezal, JM. (2012). In Vivo Scintigraphic Imaging of Proteoglycans. In: Rédini, F. (eds) Proteoglycans. Methods in Molecular Biology, vol 836. Humana Press. https://doi.org/10.1007/978-1-61779-498-8_13
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DOI: https://doi.org/10.1007/978-1-61779-498-8_13
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