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Bioluminescent Reporter Genes for Promoter Discovery

  • Tina K. Van DykEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 834)

Abstract

Discovery of promoter elements with previously unknown regulated responses is important for metabolic engineering. For example, promoters responsive to the end product can be useful to regulate expression with increasing levels of product. In addition, such promoters can be used as screens for production strain with increased titers. Use of reporter genes, such as a bioluminescent reporter luxCDABE, can facilitate promoter discovery. Here, protocols for analysis of genome-wide luxCDABE reporter gene collections in Escherichia coli are provided. Further, a protocol for using a selected para-hydroxycinnamic (pHCA)-responsive promoter as detection assay for bioproduced pHCA is provided.

Key words

Bacterial bioluminescence Luminometry lux reporter gene fusions LuxArray 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.DuPont Central Research and DevelopmentWilmingtonUSA

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