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Mutagenic Inverted Repeats Assisted Genome Engineering (MIRAGE) in Saccharomyces cerevisiae: Deletion of gal7

  • Nikhil U. Nair
  • Huimin ZhaoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 834)

Abstract

MIRAGE is a unique in vivo genome editing technique that exploits the inherent instability of inverted repeats (palindromes) in the Saccharomyces cerevisiae chromosome. As a technique able to quickly create deletions as well as precise point mutations, it is valuable in applications that require creation of designer strains of this yeast. In particular, it has various potential applications in metabolic engineering, systems biology, synthetic biology, and molecular genetics.

Key words

In vivo mutagenesis Homologous recombination Inverted repeats Synthetic biology Metabolic engineering 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Chemical and Biomolecular EngineeringUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  2. 2.Departments of Chemistry, Biochemistry, and Bioengineering, Institute for Genomic BiologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA

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