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Protein Dynamics by 15N Nuclear Magnetic Relaxation

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Protein NMR Techniques

Part of the book series: Methods in Molecular Biology ((MIMB,volume 831))

Abstract

Nitrogen-15 relaxation is the most ubiquitous source of information about protein (backbone) dynamics used by NMR spectroscopists. It provides the general characteristics of hydrodynamics as well as internal motions on subnanosecond, micro- and millisecond timescales of a biomolecule. Here, we present a full protocol to perform and analyze a series of experiments to measure the 15N longitudinal relaxation rate, the 15N transverse relaxation rate under an echo train or a single echo, the 15N–1H dipolar cross-relaxation rate, as well as the longitudinal and transverse cross-relaxation rates due to the cross-correlation of the nitrogen-15 chemical shift anisotropy and the dipolar coupling with the adjacent proton. These rates can be employed to carry out model-free analyses and can be used to quantify accurately the contribution of chemical exchange to transverse relaxation.

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Acknowledgments

I am grateful to Geoffrey Bodenhausen, David Cowburn, Ranajeet Ghose, Arthur G. Palmer, and Philippe Pelupessy for their many contributions to my training, from hands-on practice to many insightful discussions. I thank Mikael Akke for the sample of Calbindin D9k and Kaushik Dutta for carefully reading this manuscript.

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Authors and Affiliations

  1. Département de chimie, Ecole normale supérieure et Laboratoire des Biomolécules, CNRS UMR 7203, Paris, Cedex, France

    Fabien Ferrage

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  1. Fabien Ferrage
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Correspondence to Fabien Ferrage .

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Editors and Affiliations

  1. University of Albany, State University of New York, Washington Avenue 1400, Albany, 12222, New York, USA

    Alexander Shekhtman

  2. University of Albany, State University of New York, Washington Avenue 1400, Albany, 12222, New York, USA

    David S. Burz

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Ferrage, F. (2012). Protein Dynamics by 15N Nuclear Magnetic Relaxation. In: Shekhtman, A., Burz, D. (eds) Protein NMR Techniques. Methods in Molecular Biology, vol 831. Humana Press. https://doi.org/10.1007/978-1-61779-480-3_9

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  • DOI: https://doi.org/10.1007/978-1-61779-480-3_9

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-479-7

  • Online ISBN: 978-1-61779-480-3

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