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Isotope Labeling in Insect Cells

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Protein NMR Techniques

Part of the book series: Methods in Molecular Biology ((MIMB,volume 831))

Abstract

Recent years have seen remarkable progress in applying nuclear magnetic resonance (NMR) spectroscopy to proteins that have traditionally been difficult to study due to issues with folding, posttranslational modification, and expression levels or combinations thereof. In particular, insect cells have proved useful in allowing large quantities of isotope-labeled, functional proteins to be obtained and purified to homogeneity, allowing study of their structures and dynamics by using NMR. Here, we provide protocols that have proven successful in such endeavors.

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Author information

Authors and Affiliations

  1. Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe-University Frankfurt, Frankfurt am Main, Germany

    Krishna Saxena & Harald Schwalbe

  2. Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    Arpana Dutta & Judith Klein-Seetharaman

Authors
  1. Krishna Saxena
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  2. Arpana Dutta
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  3. Judith Klein-Seetharaman
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  4. Harald Schwalbe
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Corresponding author

Correspondence to Harald Schwalbe .

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Editors and Affiliations

  1. University of Albany, State University of New York, Washington Avenue 1400, Albany, 12222, New York, USA

    Alexander Shekhtman

  2. University of Albany, State University of New York, Washington Avenue 1400, Albany, 12222, New York, USA

    David S. Burz

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Saxena, K., Dutta, A., Klein-Seetharaman, J., Schwalbe, H. (2012). Isotope Labeling in Insect Cells. In: Shekhtman, A., Burz, D. (eds) Protein NMR Techniques. Methods in Molecular Biology, vol 831. Humana Press. https://doi.org/10.1007/978-1-61779-480-3_3

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  • DOI: https://doi.org/10.1007/978-1-61779-480-3_3

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-479-7

  • Online ISBN: 978-1-61779-480-3

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