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Solid-State NMR Spectroscopy of Protein Complexes

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Protein NMR Techniques

Part of the book series: Methods in Molecular Biology ((MIMB,volume 831))

Abstract

Protein–protein interactions are vital for many biological processes. These interactions often result in the formation of protein assemblies that are large in size, insoluble, and difficult to crystallize, and therefore are challenging to study by structure biology techniques, such as single crystal X-ray diffraction and solution NMR spectroscopy. Solid-state NMR (SSNMR) spectroscopy is emerging as a promising technique for studies of such protein assemblies because it is not limited by molecular size, solubility, or lack of long-range order. In the past several years, we have applied magic angle spinning SSNMR-based methods to study several protein complexes. In this chapter, we discuss the general SSNMR methodologies employed for structural and dynamics analyses of protein complexes with specific examples from our work on thioredoxin reassemblies, HIV-1 capsid protein assemblies, and microtubule-associated protein assemblies. We present protocols for sample preparation and characterization, pulse sequences, SSNMR spectra collection, and data analysis.

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Acknowledgments

The projects discussed here are supported by the National Institutes of General Medical Sciences (NIH Grants P50GM082251 and R01GM085306) and the National Center for Research Resources (NIH Grants P20RR017716-07 and P20RR015588). The authors thank Maria Luisa Tasayco, Dabeiba Marulanda, Jun Yang, Marcela Cataldi, Vilma Arriaran for their contributions to the preparation of thioredoxin reassemblies and/or solid-state NMR studies of these reassemblies.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of Delaware, Newark, DE, USA

    Shangjin Sun, Yun Han, Sivakumar Paramasivam, Si Yan & Tatyana Polenova

  2. Department of Molecular Medicine, Beckman Research Institute of City of Hope, Duarte, CA, USA

    Amanda E. Siglin & John C. Williams

  3. Department of Structural Biology, Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    In-Ja L. Byeon, Jinwoo Ahn & Angela M. Gronenborn

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  1. Shangjin Sun
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  2. Yun Han
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  3. Sivakumar Paramasivam
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  10. Tatyana Polenova
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Correspondence to Tatyana Polenova .

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Editors and Affiliations

  1. University of Albany, State University of New York, Washington Avenue 1400, Albany, 12222, New York, USA

    Alexander Shekhtman

  2. University of Albany, State University of New York, Washington Avenue 1400, Albany, 12222, New York, USA

    David S. Burz

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Sun, S. et al. (2012). Solid-State NMR Spectroscopy of Protein Complexes. In: Shekhtman, A., Burz, D. (eds) Protein NMR Techniques. Methods in Molecular Biology, vol 831. Humana Press. https://doi.org/10.1007/978-1-61779-480-3_17

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  • DOI: https://doi.org/10.1007/978-1-61779-480-3_17

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