Abstract
Previously, polyubiquitin chains have been believed to be generated through isopeptide linkages between C-terminal of carboxyl group of ubiquitin and ε-amino group of one of the seven lysine residues in another ubiquitin. In 2006, a new type of polyubiquitin chain was identified in which the C-terminal carboxyl group of one ubiquitin is conjugated to α-amino group of the N-terminal methionine of another ubiquitin. The new type of polyubiquitin was named as the linear polyubiquitin chain. Linear polyubiquitin chains were shown to be involved in NF-κB activation. Here, we describe methods to synthesize linear polyubiquitin chains in vitro and to detect linear chains in vivo.
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Acknowledgments
This work was partially supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan to K.I.
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Iwai, K. (2012). Synthesis and Analysis of Linear Ubiquitin Chains. In: Dohmen, R., Scheffner, M. (eds) Ubiquitin Family Modifiers and the Proteasome. Methods in Molecular Biology, vol 832. Humana Press. https://doi.org/10.1007/978-1-61779-474-2_16
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DOI: https://doi.org/10.1007/978-1-61779-474-2_16
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