Isolation of Adult Human Pluripotent Stem Cells from Mesenchymal Cell Populations and Their Application to Liver Damages

  • Shohei Wakao
  • Masaaki Kitada
  • Yasumasa Kuroda
  • Mari DezawaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 826)


We have found a novel type of pluripotent stem cells, Multilineage-differentiating stress enduring (Muse) cells that can be isolated from mesenchymal cell populations. Muse cells are characterized by stress tolerance, expression of pluripotency markers, self-renewal, and the ability to differentiate into endodermal-, mesodermal-, and ectodermal-lineage cells from a single cell, demonstrating that they are pluripotent stem cells. They can be isolated as cells positive for stage-specific embryonic antigen-3, a human pluripotent stem cell marker. Here, we introduce the isolation method for Muse cells and the effect of transplantation of these cells on chronic liver diseases.

Key words

Muse Pluripotent stem cells SSEA-3 Chronic liver diseases Transplantation 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Shohei Wakao
    • 1
  • Masaaki Kitada
    • 1
  • Yasumasa Kuroda
    • 1
  • Mari Dezawa
    • 1
    Email author
  1. 1.Department of Stem Cell Biology and HistologyTohoku University Graduate School of MedicineSendaiJapan

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