Development of Immortalized Hepatocyte-Like Cells from hMSCs

  • Adisak Wongkajornsilp
  • Khanit Sa-ngiamsuntorn
  • Suradej HongengEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 826)


Clones of hepatocyte-like cells were reproducibly generated from human mesenchymal stem cells immortalized with a combined transduction of both Bmi-1 and TERT genes. These hepatocyte-like cells contained selective markers and several functional properties of hepatocytes, yet still carried proliferative potential. These cells had cuboidal morphology and arranged themselves as cord-like structure in culture. The cloned cells deposited glycogen and actively synthesized albumin. The basal expressions of CYP450 isozymes was observed, albeit only 10–20% that of primary hepatocytes. These expressions were promptly increased upon the addition of rifampicin, a known enzyme inducer. These hepatocyte-like cells may serve as a close alternative to the use of primary hepatocytes for in vitro studies.

Key words

Hepatocyte-like cell hMSC Cell immortalization Hepatocyte differentiation CYP450 Drug metabolism Toxicology 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Adisak Wongkajornsilp
    • 1
  • Khanit Sa-ngiamsuntorn
    • 1
  • Suradej Hongeng
    • 2
    Email author
  1. 1.Department of Pharmacology, Faculty of Medicine Siriraj HospitalMahidol UniversityBangkokThailand
  2. 2.Department of Pediatrics, Faculty of Medicine, Ramathibodi HospitalMahidol UniversityBangkokThailand

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