Isolation of Hepatic Progenitor Cells from the Galactosamine-Treated Rat Liver
Oval cells and small hepatocytes (SHs) are well known as hepatic stem/progenitor cells. However, the relationship between the oval cells and SHs in liver regeneration is not well understood. To resolve this issue, we established a technique to selectively separate oval cells and SHs. In the injured rat liver, oval cells and SHs transiently appear in the initial period of liver regeneration. Thy1+ and CD44+ cells are candidates for markers of oval cells and SHs, respectively. In this chapter, the methods for sorting and culture of the cells are described in detail.
Key wordsOval cells Small hepatocytes Thy1 CD44 Liver Stem/progenitor cells Galactosamine
We thank Ms. Minako Kuwano and Ms. Yumiko Tsukamoto for technical assistance. We also thank Mr. Kim Barrymore for help with the manuscript. This work was supported by Grants-in-Aid for Scientific Research (for N. Ichinohe, J. Kon, and for T. Mitaka) and the program for developing the supporting system for upgrading education and research (for T. Mitaka) from the Ministry of Education, Culture, Sports, Science, and Technology, the Science and Technology Incubation Program in Advanced Regions from the Japan Science and Technology Agency (for T. Mitaka), Research on Advanced Medical Technology from the Ministry of Health, Labor, and Welfare, and Labor Sciences Research Grants (for T. Mitaka), and the Suhara Memorial Foundation (for T. Mitaka).
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