Identification and Isolation of Adult Liver Stem/Progenitor Cells

  • Minoru TanakaEmail author
  • Atsushi Miyajima
Part of the Methods in Molecular Biology book series (MIMB, volume 826)


Hepatoblasts are considered to be liver stem/progenitor cells in the fetus because they propagate and differentiate into two types of liver epithelial cells, hepatocytes and cholangiocytes. In adults, oval cells that emerge in severely injured liver are considered facultative hepatic stem/progenitor cells. However, the nature of oval cells has remained unclear for long time due to the lack of a method to isolate them. It has also been unclear whether liver stem/progenitor cells exist in normal adult liver. Recently, we and others have successfully identified oval cells and adult liver stem/progenitor cells. Here, we describe the identification and isolation of mouse liver stem/progenitor cells by utilizing antibodies against specific cell surface marker molecules.

Key words

Liver stem/progenitor cells Oval cells Flow cytometry Antibody 


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Laboratory of Cell Growth and Differentiation, Institute of Molecular and Cellular BiosciencesThe University of TokyoTokyoJapan

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